Вестник Московского университета
Серия XXIII
Антропология
Серия XXIII
Антропология
Belgorod National Research University, Belgorod, Russia
Krikun Evgeniy, e-mail: krikun@bsu.edu.ru
Among the cases of chronic dermatosis, eczema makes 30 to 40%. The rise of sickness rate, the chronicity of the disease process, the frequent recurrence of its course, insufficient effect of the treatment methods make chronic eczema the most pressing problem in the modern dermatology. At present the leading pathogenic link of eczema development is considered to be a marked immunological disorders. The main role here is played by T-lymphocytes that bear specific receptors to the antigen and discharge a number of proinflammatory cytokines as well as the factors of tumor necrosis and their receptors. In connection with this, the object of this research work is the study of the role of genetic polymorphism +1663A/G TNFR2 in the development of chronic true eczema. 363 persons were examined (58 patients and 305 persons of the control group), all of Russian nationality, natives of the Central Chernozem region of the RF and having no blood relationship. The extraction of genome DNA from peripheral blood is made with the method of phenol-chlorophorm extraction. PCR was made on the amplifier IQ 5 (Bio-Rad) in the real time mode of operation with the use of DNA polymerases Thermus aquaticus and oligonucleotide primers and probes. Genotyping of DNA markers is made with the method of allele discrimination on the base of Tag Man probes. The results of the research revealed the following rates of genotypes among the patients with chronic true eczema: +1663 AA – 6.9%; +1663 AG – 63.8%; +1663GG – 29.3%, and the rates of alleles are as follows +1663A – 38.79%; +1663G – 61.21. The rates of the genotypes in the control group were the following: +1663AA – 21.64%; +1663 AG – 45.24%; +1663GG – 33.12% and the rates of alleles were: +1663A – 44.26%; +1663G – 55.74%. According to this comparative analysis, the statistical significant differences in the concentration of alleles and genotypes of this locus are not revealed in the group of patients and in the control group. This allows us to come to a conclusion that the polymorphism +1663A/G TNFR2 is not associated with the development of chronic true eczema.
eczema, genetic polymorphism, natives of the Central Chernozem region of Russia
Цит.: Denisova Ya., Chournosov M., Krikun E. INFLUENCE OF GENETIC POLYMORPHISM +1663A/G TNFR2 ON THE DEVELOPMENT OF CHRONIC TRUE ECZEMA // Вестник Московского университета. Серия XXIII. Антропология, 2014; 3/2014; с. 118-118
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